diff --git a/homeassistant/components/profiler/__init__.py b/homeassistant/components/profiler/__init__.py
index 389e3384ad9b902737d44feab9caf3070e568969..9b2b97365746e9b533e8ab8d7dad948fb89a756e 100644
--- a/homeassistant/components/profiler/__init__.py
+++ b/homeassistant/components/profiler/__init__.py
@@ -436,10 +436,6 @@ async def _async_generate_memory_profile(hass: HomeAssistant, call: ServiceCall)
     # Imports deferred to avoid loading modules
     # in memory since usually only one part of this
     # integration is used at a time
-    if sys.version_info >= (3, 13):
-        raise HomeAssistantError(
-            "Memory profiling is not supported on Python 3.13. Please use Python 3.12."
-        )
     from guppy import hpy  # pylint: disable=import-outside-toplevel
 
     start_time = int(time.time() * 1000000)
diff --git a/homeassistant/components/profiler/manifest.json b/homeassistant/components/profiler/manifest.json
index 8d2814c8c7f58b78c8d8cb911b3b3fc399f5dd11..814b00a16d4702b81d506d248c0206a5260a3e37 100644
--- a/homeassistant/components/profiler/manifest.json
+++ b/homeassistant/components/profiler/manifest.json
@@ -7,7 +7,7 @@
   "quality_scale": "internal",
   "requirements": [
     "pyprof2calltree==1.4.5",
-    "guppy3==3.1.4.post1;python_version<'3.13'",
+    "guppy3==3.1.5",
     "objgraph==3.5.0"
   ],
   "single_config_entry": true
diff --git a/requirements_all.txt b/requirements_all.txt
index 30f96e366aa584575661c60635e6675b71724725..2f05bba18ac8fae7cf5a72b18e99eab449d29984 100644
--- a/requirements_all.txt
+++ b/requirements_all.txt
@@ -1079,7 +1079,7 @@ gspread==5.5.0
 gstreamer-player==1.1.2
 
 # homeassistant.components.profiler
-guppy3==3.1.4.post1;python_version<'3.13'
+guppy3==3.1.5
 
 # homeassistant.components.iaqualink
 h2==4.1.0
diff --git a/requirements_test_all.txt b/requirements_test_all.txt
index d50cf3be694c2ac9919de82436311b9b20fe92aa..7271b97c812845bd7d5a8c71f0ea38fa08307880 100644
--- a/requirements_test_all.txt
+++ b/requirements_test_all.txt
@@ -920,7 +920,7 @@ growattServer==1.5.0
 gspread==5.5.0
 
 # homeassistant.components.profiler
-guppy3==3.1.4.post1;python_version<'3.13'
+guppy3==3.1.5
 
 # homeassistant.components.iaqualink
 h2==4.1.0
diff --git a/tests/components/profiler/test_init.py b/tests/components/profiler/test_init.py
index 84314b7b22cbc61364e83d0c53e8fb04296116b3..540e644aca40b49b3f1e11bd0030280c1129f7ef 100644
--- a/tests/components/profiler/test_init.py
+++ b/tests/components/profiler/test_init.py
@@ -5,7 +5,6 @@ from functools import lru_cache
 import logging
 import os
 from pathlib import Path
-import sys
 from unittest.mock import patch
 
 from freezegun.api import FrozenDateTimeFactory
@@ -71,9 +70,6 @@ async def test_basic_usage(hass: HomeAssistant, tmp_path: Path) -> None:
     await hass.async_block_till_done()
 
 
-@pytest.mark.skipif(
-    sys.version_info >= (3, 13), reason="not yet available on Python 3.13"
-)
 async def test_memory_usage(hass: HomeAssistant, tmp_path: Path) -> None:
     """Test we can setup and the service is registered."""
     test_dir = tmp_path / "profiles"
@@ -105,24 +101,6 @@ async def test_memory_usage(hass: HomeAssistant, tmp_path: Path) -> None:
     await hass.async_block_till_done()
 
 
-@pytest.mark.skipif(sys.version_info < (3, 13), reason="still works on python 3.12")
-async def test_memory_usage_py313(hass: HomeAssistant, tmp_path: Path) -> None:
-    """Test raise an error on python3.13."""
-    entry = MockConfigEntry(domain=DOMAIN)
-    entry.add_to_hass(hass)
-
-    assert await hass.config_entries.async_setup(entry.entry_id)
-    await hass.async_block_till_done()
-    assert hass.services.has_service(DOMAIN, SERVICE_MEMORY)
-    with pytest.raises(
-        HomeAssistantError,
-        match="Memory profiling is not supported on Python 3.13. Please use Python 3.12.",
-    ):
-        await hass.services.async_call(
-            DOMAIN, SERVICE_MEMORY, {CONF_SECONDS: 0.000001}, blocking=True
-        )
-
-
 async def test_object_growth_logging(
     hass: HomeAssistant,
     caplog: pytest.LogCaptureFixture,